New polymerases for nucleic acid amplification and sequencing

POSTED : 17/09/2013

CATEGORIES : Life Sciences

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Short Description

Our company is looking for new polymerases that could be used in DNA sequencing and amplification, that overcome the constraints in currently marketed polymerases. The world of medicine is shifting from the traditional "one size fits all" approach to one where the genetic profile of patients is used to make the most effective clinical decisions for individual patients – a concept known as Personalized Medicine. Personalized Medicine is a new important driver for the polymerase market. Personalized Medicine tries to tailor medical diagnostics and treatments to the individual characteristics of the patients, i.e., their genetic repertoire. Automated systems for sequencing DNA or spot-checking for genetic variation at low costs are essential to progress in both research and clinical applications, and this is pushing forward the need for new polymerases adapted to the so called Next Generation Sequencing equipments. A DNA polymerase is a cellular or viral polymerase enzyme that synthesizes DNA molecules from their nucleotide building blocks. DNA polymerases are essential for DNA replication Nowadays, there is an increasing need for improved polymerases, mainly Phi29 Pol based, in today’s demanding sequencing applications. Current polymerases used have problems with modified nucleotide handling etc. that have to be overcome. There is a considerable market need for improved amplification and sequencing enzymes due to frequent problems with reading lengths and error rates in today’s sequencing technologies.

Organization

SME

Country

Germany

Grade of development

Proof of Concept

Collaboration Sought

Joint further development

Desired outcome of the collaboration

Our aim is to license-in or develop novel polymerases for next generation sequencing and nucleic acid amplification. Both targeted mutations and directed mutagenesis will be used to improve properties (reduced bias, higher yields in amplification, higher modified nucleotide tolerance and fidelity of incorporation, reduced exonuclease activity for sequencing applications in Phi29 DNA and other polymerases).

Partners with experience on this field from industry and academy are welcomed to send their expression of interest in order to evaluate and negotiate one of the following potential agreements:

1.     in-licensing agreement

2.     Joint further development agreement for discovering, characterized or engineering such new polimerases

Topics

molecular biology,DNA sequencing,DNA amplification,polymerases

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